鎮ㄦ墍鍦ㄤ綅缃細棣栭〉/浜у搧涓績

纾烽吀鍖栬泲鐧藉垎绂籔hos-tagTM绯诲垪浜у搧

Phos-tagTM绯诲垪浜у搧鍒╃敤鑳戒笌纾烽吀鍒嗗瓙鐗瑰紓鎬х粨鍚堢殑Phos-tagTM鍒嗗瓙锛屾棤闇€鏀惧皠鎬х墿璐ㄣ€佸寲瀛︾墿璐ㄦ爣绛句笌鎶楃7閰稿寲鎶椾綋鍗冲彲鐗瑰紓鎬ф崟鑾风7閰稿熀鍥紝鍙敤浜庣7閰稿寲铔嬬櫧鐨勫垎绂伙紝閫傚悎妞嶇墿铔嬬櫧纾烽吀鍖栫瓑棰嗗煙涓殑铔嬬櫧纾烽吀鍖栫爺绌讹紒

鏌ョ湅璇︽儏

Trypsin Gold, Mass Spectrometry Grade

鑳拌泲鐧介叾 (Trypsin) 鏄笣姘ㄩ吀铔嬬櫧閰讹紝鍙壒寮傛€у湴鍒囧紑璧栨皑閰稿拰绮炬皑閰告畫鍩虹殑缇у熀绔€傝儼铔嬬櫧閰惰繖绉嶄弗鏍肩殑鐗瑰紓鎬ф槸铔嬬櫧璐ㄩ壌瀹氱殑鍩虹銆傚ぉ鐒惰儼铔嬬櫧閰舵槗浜庤嚜鎴戞按瑙o紝浜х敓鍋囪儼铔嬬櫧閰讹紝鍚庤€呯壒寮傛€ц緝骞匡紝鍚湁鑳板嚌涔宠泲鐧介叾鏍锋椿鎬с€傚瓨鍦ㄤ簬鑳拌泲鐧介叾鍒跺墏涓殑杩欎簺鑷垜姘磋В浜х墿鑳戒骇鐢熷浣欑殑鑲界墖娈碉紝浠庤€屽共鎵拌川璋辨娴嬪埌鐨勭墖娈电殑鏁版嵁鍒嗘瀽銆俆rypsin Gold, Mass Spectrometry Grade ( 鑳拌泲鐧介叾锛岃川璋卞垎鏋愮骇 ) 鑳芥彁渚涙渶澶ч檺搴︾殑鐏垫晱搴︺€傜尓鑳拌泲鐧介叾涓殑璧栨皑閰告畫鍩哄凡缁忚繃杩樺師鐢插熀鍖栦慨楗帮紝鎴愪负娲绘€ч珮涓旂ǔ瀹氱殑鍒嗗瓙锛屾姉鎷掕嚜鎴戞按瑙g殑鎬ц兘鐗瑰埆寮恒€ 绾寲鐨勮儼铔嬬櫧閰剁殑鐗瑰紓鎬у張鐢变簬 TPCK 澶勭悊浣胯儼鍑濅钩铔嬬櫧閰跺け娲昏€岃繘涓€姝ユ彁楂樸€傚鐞嗚繃鐨勮儼铔嬬櫧閰跺張缁忚繃浜插拰灞傛瀽绾寲锛屽埗鎴愬喕骞茬矇锛屾垚涓 Trypsin Gold, Mass Spectrometry Grade銆傝儼铔嬬櫧閰跺父鐢ㄤ簬鑳跺唴娑堝寲銆傛秷鍖栦骇鐗╃粡绾寲鍜屾祿缂╋紝鐒跺悗鍐嶈繘琛岃川璋卞垎鏋愮‘瀹氬垎瀛愰噺銆傜劧鍚庣敤鑲芥鐨勮川閲忔悳绱㈡暟鎹簱锛岄壌瀹氬垎绂诲湪鑳朵腑鐨勮泲鐧借川銆備骇鍝佽€愬彈杞诲害鍙樻€ф潯浠 ( 濡 0.1% SDS锛?M 灏跨礌鎴 10% 涔欒厛 )锛屽苟鍦 2M 鐩愰吀鑳嶄腑浠嶄繚鐣 50% 鐨勬椿鎬с€傚綋閰告€ф畫鍩哄瓨鍦ㄤ簬鏄撴劅閿换涓€渚ф椂锛岃儼铔嬬櫧閰剁殑娲绘€т細闄嶄綆銆傚鏋滆劘姘ㄩ吀瀛樺湪浜庤禆姘ㄩ吀鎴栫簿姘ㄩ吀鐨勭晶鍩虹锛屽舰鎴愮殑鑲介敭鍑犱箮瀹屽叏鎶楄儼铔嬬櫧閰跺垏鍓层€傛瘡鎵圭粡杩囪川妫€鐨 Trypsin Gold, Mass Spectrometry Grade 鑳藉淇濋殰杩涜鑳跺唴娑堝寲鍜岃川璋卞垎鏋愩€侟/p> 鏌ョ湅璇︽儏

RIPA Lysis Buffer

娓╁拰楂樻晥-涓嶅惈 SDS 鐨勬俯鍜岄厤鏂癸紝鍏呭垎鎶芥彁鍙憾铔嬬櫧 鎬т环姣旈珮-Cat#20-188 浠 10脳娴撶缉褰㈠紡鎻愪緵锛屽崟娆¤瑙h垂鐢ㄨ繙浣庝簬鍚岀被杩涘彛浜у搧鍜屽浗浜т骇鍝併€係igma 鐨 R0278 鍒跺鐨勮泲鐧戒繚鐣欎簡寰堝ソ鐨勫厤鐤弽搴旀€у拰鐢熺墿娲绘€э紝骞朵笖鍦ㄥ厤鐤矇娣€鍜 pull-down 涓噺灏戦潪鐗瑰紓鎬х粨鍚堜粠鑰岄檷浣庤儗鏅€ 鍏煎鎬уソ-鎶芥彁铔嬬櫧浜х墿閫傜敤浜 IP 鍜 WB 绛変笅娓稿簲鐢?/p> 鏌ョ湅璇︽儏

鑳跺師閰 Collagenase

鑳跺師閰跺張绉拌兌鍘熻泲鐧芥按瑙i叾(Collagenase)锛屽畠鑳藉湪鐢熺悊 PH 鍜屾俯搴︽潯浠朵笅鐗瑰紓鎬у湴姘磋В澶╃劧鑳跺師铔嬬櫧鐨勪笁缁磋灪鏃嬬粨鏋勶紝鑰屼笉鎹熶激鍏跺畠铔嬬櫧鍜岀粍缁囥€傝兌鍘熼叾瀵规俯搴︺€丳H 鍜屽鑷磋泲鐧借川鍙樻€х殑鍚勭鍥犵礌鍧囬潪甯告晱鎰燂紝鏋佹槗鍙楀埌澶栫晫鏉′欢鐨勫奖鍝嶈€屾敼鍙樺叾鏈韩鐨勬瀯璞″拰鎬ц川銆侟/p> 鏌ョ湅璇︽儏

鍏徃鍚嶇О锛氭箹鍗楁櫠绉戠敓鐗╃鎶€鏈夐檺鍏徃

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